abstract：:The N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP)-OMe (1) analogues for-Thp-Leu-Ain-OMe (2), for-Thp-Leu-Phe-OMe (3), for-Met-Leu-Ain-OMe (4), for-Met-Delta(z)Leu-Phe-OMe (5), for-Met-Lys-Phe-For-Met-Lys-Phe (6), for-Met-Leu-Pheol-COMe (7), and for-Nle-Leu-Phe-OMe (8) have been studied. Some of these have been ...

journal_title：Cellular signalling

authors： Ferretti ME,Nalli M,Biondi C,Colamussi ML,Pavan B,Traniello S,Spisani S

更新日期：2001-04-01 00:00:00

abstract：:The type III transmembrane adaptor protein linker for activation of T cells (LAT) is essential for membrane recruitment of signalling molecules following TCR activation. Here we show that although LAT deleted in the transmembrane domain is completely soluble, it can be tyrosine phosphorylated after anti-CD3 stimulatio...

journal_title：Cellular signalling

authors： Torgersen KM,Vaage JT,Rolstad B,Taskén K

更新日期：2001-03-01 00:00:00

abstract：:Extracellular adenine dinucleotides are modulators of blood vessel tone. We have previously demonstrated that Ap(2)A and Ap(4)A induce the synthesis of nitric oxide (NO) from bovine aortic endothelial cells (BAEC) while Ap(3)A and Ap(5)A do not [FEBS Lett. 427 (1998) 320; Arch. Biochem. Biophys. 364 (1999) 280.]. In t...

journal_title：Cellular signalling

authors： Haghiac M,Pojoga LH,Hilderman RH

更新日期：2001-02-01 00:00:00

abstract：:In this work, we used colon carcinoma cell-line HCT116 to study the involvement of the 86-kDa subunit (Ku86) of DNA-protein kinase (DNA-PK) in human tumoural cell proliferation. We transfected these cells with a 639-bp cDNA encoding a Ku86 portion inserted into pcDNA3.1 vector in the antisense orientation. After selec...

journal_title：Cellular signalling

authors： Sadji Z,Le Romancer M,Lewin MJ,Reyl-Desmars F

更新日期：2000-12-01 00:00:00

abstract：:A reduced capacity for insulin to elicit increases in glucose uptake and metabolism in target tissues such as skeletal muscle is a common feature of obesity and diabetes. The association between lipid oversupply and such insulin resistance is well established, and evidence for mechanisms through which lipids could pla...

journal_title：Cellular signalling

authors： Schmitz-Peiffer C

更新日期：2000-10-01 00:00:00

abstract：:In vitro, little specificity is seen for modulation of effectors by different combinations of Gbetagamma subunits from heterotrimeric G proteins. Here, we demonstrate that the coupling of specific combinations of Gbetagamma subunits to different receptors leads to a differential ability to modulate effectors in vivo. ...

journal_title：Cellular signalling

authors： Robillard L,Ethier N,Lachance M,Hébert TE

更新日期：2000-10-01 00:00:00

abstract：:We demonstrate the difference between the follistatin isoforms (FS-288 and FS-315), two activin-binding proteins, in the neutralizing activity for activin signalling. Transcriptional reporter assay using 3TP-Lux, an activin-responsive reporter construct, showed that the inhibitory effect of FS-288 on activin-induced t...

journal_title：Cellular signalling

authors： Hashimoto O,Kawasaki N,Tsuchida K,Shimasaki S,Hayakawa T,Sugino H

更新日期：2000-08-01 00:00:00

abstract：:Extracellular signal-regulated kinase (ERK)-dependent phosphorylation is an important regulator for cytosolic phospholipase A(2) (cPLA(2)). In this study, we found that the protein synthesis inhibitor cycloheximide can potentiate thapsigargin-induced arachidonic acid (AA) release concomitant with ERK phosphorylation f...

journal_title：Cellular signalling

authors： Lin WW,Hsu YW

更新日期：2000-07-01 00:00:00

abstract：:The cyclin-dependent kinase inhibitors (CKI) interact with cyclin-cdk complexes to arrest mitogen-stimulated transit through the cell cycle, but we and others have recently shown that these molecules can exert permissive effects on cell cycle transit as well. The p53 protein induces transcription of the p21(Waf1/Cip1)...

journal_title：Cellular signalling

authors： Weiss RH,Randour CJ

更新日期：2000-06-01 00:00:00

abstract：:The roles of protein kinase C (PKC) isoenzymes in the differentiation process of THP-1 cells are investigated. Inhibition of PKC by RO 31-8220 reduces the phagocytosis of latex particles and the release of superoxide, prostaglandin E(2) (PGE(2)), and tumour necrosis factor (TNF)-alpha. The proliferation of THP-1 cells...

journal_title：Cellular signalling

authors： Dieter P,Schwende H

更新日期：2000-05-01 00:00:00

abstract：:We have previously shown that N18TG2 neuroblastoma cells express the type 6 adenylyl cyclase and that preincubation with nitric oxide (NO) attenuates Gs- and forskolin-stimulated activity. Here we show that this inhibition reflects a direct action of NO on the adenylyl cyclase. Preincubation of N18TG2 cell membranes a...

journal_title：Cellular signalling

authors： Hill J,Howlett A,Klein C

更新日期：2000-04-01 00:00:00

abstract：:Activation of phosphatidylinositol (PI)-kinase is involved in the regulation of a wide array of cellular activities. The enzyme exists as a dimer, consisting of a catalytic and a regulatory subunit. Five isoforms of the regulatory subunit have been identified and classified into three groups comprising respectively 85...

journal_title：Cellular signalling

authors： Funaki M,Katagiri H,Inukai K,Kikuchi M,Asano T

更新日期：2000-03-01 00:00:00

abstract：:Heterotrimeric (alphabetagamma) G(s) mediates agonist-induced stimulation of adenylyl cyclase (AC). Cholera toxin (CTx) will ADP-ribosylate the alpha-subunit of G(s) (G(s)alpha). G(s)alpha-deficient cyc(-) membranes were "stripped" of Gbeta. When the stripped cyc(-) were incubated with G(s)alpha and/or Gbetagamma, eac...

journal_title：Cellular signalling

authors： Ganpat MM,Nishimura M,Toyoshige M,Okuya S,Pointer RH,Rebois RV

更新日期：2000-02-01 00:00:00

abstract：:The Wnt signalling pathway is conserved in various species from worms to mammals, and plays important roles in development, cellular proliferation, and differentiation. The molecular mechanisms by which the Wnt signal regulates cellular functions are becoming increasingly well understood. Wnt stabilizes cytoplasmic be...

journal_title：Cellular signalling

authors： Kikuchi A

更新日期：1999-11-01 00:00:00

abstract：:Epidermal growth factor (EGF) and transforming growth factor type beta1 (TGF-beta1) exert opposite effects in most cells. A potential regulation between the two factors has been studied at a transcriptional level, but never at a protein level. MDA-MB-231 is a breast carcinoma cell line which possesses large quantities...

journal_title：Cellular signalling

authors： Martínez-Carpio PA,Mur C,Rosel P,Navarro MA

更新日期：1999-10-01 00:00:00

abstract：:The implication of MAP kinases in the proliferation control of pancreatic cancer cells is still unknown. This study was undertaken to examine the contribution of the p44/p42 and p38 MAP kinases in the mitogenic response to epidermal growth factor (EGF) and bombesin in human pancreatic cancer cells, MIA PaCa-2 and PANC...

journal_title：Cellular signalling

authors： Douziech N,Calvo E,Lainé J,Morisset J

更新日期：1999-08-01 00:00:00

abstract：:Somatostatin receptors (SSTRs) are known to mediate diverse cellular responses. Most target cell express more than one SSTR isoform, making it difficult to define the signalling pathway used by individual receptor subtypes. Thus, we have expressed SSTR1 or SSTR2 in rat pituitary F4C1 cells which lack endogenous SSTRs....

journal_title：Cellular signalling

authors： Chen L,Tashjian AH Jr

更新日期：1999-07-01 00:00:00

abstract：:The effect of 5-hydroxytryptamine (5-HT) on phospholipase C (PLC)-mediated phosphoinositide (PI) hydrolysis and intracellular Ca2+ ([Ca2+]i) changes was investigated in canine cultured aorta smooth muscle cells (ASMCs). 5-HT-stimulated inositol phosphate (IP) accumulation was time and concentration dependent with a ha...

journal_title：Cellular signalling

authors： Chiu CT,Tsao HL,Fan LW,Wang CC,Chien CS,Yang CM

更新日期：1999-05-01 00:00:00

abstract：:Src homology (SH) 3 domains are small modules found in a diverse array of proteins. The presence of an SH3 domain confers upon its resident protein the ability to interact with specific proline-rich sequences in protein binding partners. A major focus of research has highlighted a role for SH3 domain-mediated interact...

journal_title：Cellular signalling

authors： McPherson PS

更新日期：1999-04-01 00:00:00

abstract：:PGE2 and prostacyclin each enhance cAMP synthesis in the osteoblast-like cell line UMR-106. The amount of cAMP induced by PGE2 was 5-7-fold greater than the amount induced by cicaprost or iloprost, stable prostacyclin analogues. Both PGE2 and the two prostacyclin analogues enhanced cAMP synthesis with similar time dep...

journal_title：Cellular signalling

authors： Khanin M,Liel Y,Rimon G

更新日期：1999-03-01 00:00:00

abstract：:Cdc25 and Ras are two proteins required for cAMP signalling in the budding yeast Saccharomyces cerevisiae. Cdc25 is the guanine nucleotide exchange protein that activates Ras. Ras, in turn, activates adenylyl cyclase. Cdc25 has a Src homology 3 (SH3) domain near the N-terminus and a catalytic domain in the C-terminal ...

journal_title：Cellular signalling

authors： Mintzer KA,Field J

更新日期：1999-02-01 00:00:00

abstract：:Activation of the caspase proteases by c-Jun N-terminal kinase 1 (JNK1) has been proposed as a mechanism of apoptotic cell death. Here we report that insulin activates caspase-3 by a pathway requiring phosphatidylinositol 3'-kinase (PI3-kinase). JNK1 assays demonstrated that insulin treatment of myeloma cells induced ...

journal_title：Cellular signalling

authors： Godbout JP,Cengel KA,Cheng SL,Minshall C,Kelley KW,Freund GG

更新日期：1999-01-01 00:00:00

abstract：:Mechanical stretch is an initial factor for cardiac hypertrophy in response to haemodynamic overload (high blood pressure). Stretch of cardiomyocytes activates second messengers such as phosphatidylinositol, protein kinase C, Raf-1 kinase and extracellular signal-regulated protein kinases (ERKs), which are involved in...

journal_title：Cellular signalling

authors： Yamazaki T,Komuro I,Yazaki Y

更新日期：1998-11-01 00:00:00

abstract：:Understanding how cells integrate signals from a variety of chemically diverse information-containing molecules into complex, orchestrated responses such as cell proliferation, differentiation and apoptosis is an overarching goal of cell biology. The ligand molecules that act upon cell surface receptors include those ...

journal_title：Cellular signalling

authors： Malbon CC,Karoor V

更新日期：1998-09-01 00:00:00

abstract：:Induction of apoptosis in Hep3B hepatoma cells by transforming growth factor beta 1 (TGF-beta 1) was accompanied by the activation of interleukin-1-beta-converting-enzyme-like proteases, which have recently been renamed as caspases. The caspase inhibitor ZVAD-FMK, which has a broader specificity for caspase family pro...

journal_title：Cellular signalling

authors： Hung WC,Chang HC,Chuang LY

更新日期：1998-07-01 00:00:00

abstract：:Chbl, a 120-kDa proto-oncogene product, whose gene was first identified as part of a transforming gene of a murine retrovirus and whose expression is predominant in haematopoietic cells, consists of an amino-terminal transforming region, a zinc Ring finger, multiple proline-rich stretches, and several potential phosph...

journal_title：Cellular signalling

authors： Liu YC,Altman A

更新日期：1998-06-01 00:00:00

abstract：:Platelet-derived growth factor (PDGF) stimulates cyclic AMP (cAMP) synthesis in cultured guinea-pig airway smooth muscle (ASM) cells. However, this stimulation is normally countered by the action of cAMP phosphodiesterases. Thus, cAMP synthesis was observed only in cells pre-treated with either 3-isobutyl-1-methylxant...

journal_title：Cellular signalling

authors： Pyne NJ,Pyne S

更新日期：1998-05-01 00:00:00

abstract：:The phosphatidylinositol (PI) 3-kinase family of enzymes is now known to be regulated by several different upstream pathways in response to virtually all growth factors and cytokines. In the past few years, the phosphoinositides phosphorylated at the 3-OH position of the inositol ring have been shown to be lipid secon...

journal_title：Cellular signalling

authors： Duronio V,Scheid MP,Ettinger S

更新日期：1998-04-01 00:00:00

abstract：:We analyse the role of the G proteins in regulating collagen gene expression by measuring collagen alpha 1(I) mRNA levels in cultured hepatic stellate cells in basal conditions and after stimulating or inhibiting the major intracellular signalling pathways. Stimulation of Gs protein and adenylyl cyclase or the additio...

journal_title：Cellular signalling

authors： Solís-Herruzo JA,Hernández I,De la Torre P,García I,Sánchez JA,Fernández I,Castellano G,Muñoz-Yagüe T

更新日期：1998-03-01 00:00:00

abstract：:13-(S)-Hydroxyoctadecadienoic acid (13-HODE), the lipoxygenase metabolite of linoleic acid, has been shown to reverse the epidermal hyperproliferation induced by topical application of docosahexaenoic acid (DNA, 22:6 n-3) on guinea pig skin. Our initial studies demonstrated that 13-HODE exerts a selective inhibition o...

journal_title：Cellular signalling

authors： Mani I,Iversen L,Ziboh VA

更新日期：1998-02-01 00:00:00

abstract：:The inwardly rectifying K+ channels of the GIRK (Kir3) family, members of the superfamily of inwardly rectifying K+ channels (Kir), are important physiological tools to regulate excitability in heart and brain by neurotransmitters, and the only ion channels conclusively shown to be activated by a direct interaction wi...

journal_title：Cellular signalling

authors： Dascal N

更新日期：1997-12-01 00:00:00

abstract：:Dynamin, a 100 kD GTPase, is necessary for the normal development and function of mammalian neural tissue. In neurons, it is necessary for the biogenesis of synaptic vesicles, and in other cell types dynamin has a general and important role in clathrin mediated receptor endocytosis. Different isoforms function as mole...

journal_title：Cellular signalling

authors： Scaife RM,Margolis RL

更新日期：1997-09-01 00:00:00

abstract：:To investigate whether G protein-coupled receptor kinases (GRKs) are involved in the regulation of the PTH/PTHrPR, we have established mutant SaOS-2 cells which stably overexpress (> 10-20-fold) a dominant negative form of the beta-adrenergic receptor kinase-1 (beta ARK-1). Acute (< or = 2 h) incubation with hPTH (1-3...

journal_title：Cellular signalling

authors： Fukayama S,Kong G,Benovic JL,Meurer E,Tashjian AH Jr

更新日期：1997-09-01 00:00:00

abstract：:Strategies which can be used to elucidate the nature of a GTP-binding regulatory protein (G-protein) involved in an intracellular pathway of interest in the complex environment of the cell are described and evaluated. A desirable strategy is considered to be one in which the first stage indicates a requirement for one...

journal_title：Cellular signalling

authors： Barritt GJ,Gregory RB

更新日期：1997-05-01 00:00:00

abstract：:Transforming growth factory beta (TGF-beta) is a potent growth inhibitor of epithelial cells. One of the strategies used to elucidate the anti-proliferative mode of action of TGF-beta is to find out whether the receptor-generated signals interact with components of the basic machinery of the cell cycle. In this study ...

journal_title：Cellular signalling

authors： Landesman Y,Bringold F,Milne DD,Meek DW

更新日期：1997-05-01 00:00:00

abstract：:Caffeine and ryanodine triggered an increase in [Ca2+]i (73 +/- 22 and 61 +/- 18 nM, respectively) in Jurkat cell populations that was independent of external Ca2+. In individual cells, caffeine and ryanodine induced Ca2+ spikes. Jurkat cell populations initially exposed to caffeine did not respond further to ryanodin...

journal_title：Cellular signalling

authors： Ricard I,Martel J,Dupuis L,Dupuis G,Payet MD

更新日期：1997-02-01 00:00:00

abstract：:Previous studies implicating a role for protein kinase C (PKC) in mediating stimulation of cellular responses by physiological agonists have relied on use of non-specific inhibitors or direct stimulation of PKC by phorbol esters. However, much of this evidence is questionable. Here, we have investigated the effects of...

journal_title：Cellular signalling

authors： Merritt JE,Sullivan JA,Tse J,Wilkinson Se,Nixon JS

更新日期：1997-01-01 00:00:00

abstract：:The strong inhibition of thrombin-induced platelet functions induced by okadaic acid is not correlated with the partial modification of pleckstrin phosphorylation, which remains still phosphorylated two min after stimulation, indicating that protein kinase C is not affected by okadaic acid. We then investigated the ef...

journal_title：Cellular signalling

authors： Mauco G,Artçanuthurry V,Pidard D,Grelac F,Maclouf J,Lévy-Tolédano S

更新日期：1997-01-01 00:00:00

abstract：:In extracts of human platelets, three isoenzymes of cyclic nucleotide phosphodiesterase (PDE), namely, PDE2, PDE3, and PDE5, were identified; activities of PDE1 and PDE4 were not detected. In human platelets, the cGMP-hydrolytic activity was about six times higher than the cAMP-hydrolytic activity, and PDE5 and PDE3 a...

journal_title：Cellular signalling

authors： Ito M,Nishikawa M,Fujioka M,Miyahara M,Isaka N,Shiku H,Nakano T

更新日期：1996-12-01 00:00:00

abstract：:Protein-tyrosine phosphatases (PTPases) play an essential role in the regulation of reversible tyrosine phosphorylation of cellular proteins that mediate insulin action. In order to explore the potential role of the transmembrane PTPase (LAR) in insulin receptor signal transduction, we overexpressed the full-length LA...

journal_title：Cellular signalling

authors： Li PM,Zhang WR,Goldstein BJ

更新日期：1996-11-01 00:00:00